Convergence studies of the numerical procedures applied in establishing the calibration relations in gel permeation chromatography
Convergence studies of the numerical procedures applied in establishing the calibration relations
in gel permeation chromatography
Using the Gaussian model of GPC-chromatogram shape it bas been proved theoretically that the iterative calibration procedur es developer by the Author for establishing the calibration relations for polymolecular polymer standards are convergent. On an example of the results of measurements for polystyrene standards with a narrow and a broad molecular mass distribution it has been stated that a decisive influence on the obtained values of the calibration parameters have both the errors in the determination of the average molecular masses of standards and the chosen model of the instrumental spreading function. It is not possible to speak about the „true” values of a and К in the [h] - M relationship for a Niven polymer-solvent system at a defined temperature because of unavoidable effects encountered during their determination. The separameters have an„effective”character, what means that they can be used for the calculation of correct values of molecular masses from chromatograms with the use of the measured values of [h].
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