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M. Lewandowska-Szumieł, Z. Zimek, D. Kudelska, M. Mazur

Evaluation of the toxicity of radiosterilized implantable materials

Polimery 1997, No 3, 195

DOI: dx.doi.org/10.14314/polimery.1997.195


Autoclave and radiation sterilization modes of selected biomaterials and polymers were studied to evaluate the toxicity, if any, induced in the cells grown invitro. The materials examined included: crystalline and amorphous biocarbon, alumina, hydroxyapatite, powdered primary PP (radiation-sensitive), and a PP modified with a propylene/ethylene or an ethylene/vinyl acetate copolymer to enhance its radiation resistance. Each PP was tested for toxicity in two days and, additionally, in one month after the sample had been radiosterilized, i.e., in the latter case when structural alterations induced by radiation in a nonmodified PP become already manifest via downgraded mechanical properties. The standard MTT viability assay was applied to two types of cells, viz., mouse 3T3 fibroblasts and isolated mouse peritoneal macrophages. The media tested were placed in the 96 wells of a plate (cf. Figs. 1, 2). Results (Fig. 3) showed no material to be toxic toward the cells examined. The viability of the cells cultivated in the presence of the materials examined was found to remain unaffected regardless of the sterilization mode.

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