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P. Minkiewicz, J. Dziuba, D. Nałęcz

Application of derivatives of UV spectra and similarity indices to identification

of milk proteins separated using reversed-phase high performance

liquid chromatography (in English)

Polimery 2003, No 2, 106


Summary

The method which may be applied as a tool for identification of proteins separated using reversed-phase high performance liquid chromatography, based on similarity indices of UV spectra derivatives, was proposed. Commercial milk protein preparations such as sodium and calcium caseinates, whey protein concentrate and milk protein coprecipitate as well as standards of milk proteins were used in the experiment. Statistical significance of difference between first and second similarity index, evaluated with Student?s t test was used as a criterion to choose the mathematical treatment of spectra as well as to predict reliability of identification. First derivatives of spectra revealed the highest value of t parameter. The presence of statistically significant difference between first and second similarity indices always led to correct identification of proteins. The presented method enabled appropriate identification of caseins in commercial milk protein preparations, but failed in the case of whey proteins.


Keywords: identification of proteins, reversed-phase HPLC, UV spectroscopy, similarity indices of UV spectra derivatives


P. Minkiewicz, J. Dziuba, D. Nałęcz

Application of derivatives of UV spectra and similarity indices to identification of milk proteins separated using reversed-phase high performance liquid chromatography (in English)